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008 080804s2009 nyua b 001 0 eng
010 _a 2008934544
015 _aGBA894565
_2bnb
016 7 _a014681048
_2Uk
020 _a9781934115626 (hbk.)
020 _a1934115622 (hbk.)
020 _a9781597455237 (ebk.)
020 _a1597455237 (ebk.)
035 _a(OCoLC)ocn233933530
040 _aUKM
_cUKM
_dBTCTA
_dBAKER
_dYDXCP
_dCUS
_dCDX
_dCOU
_dDLC
042 _aukblcatcopy
_alccopycat
050 0 0 _aQP471
_b.A93 2009
060 4 _aWV 270 A912 2009
082 0 4 _a612.858 SOK
_222
245 0 0 _aAuditory and vestibular research :
_bmethods and protocols /
_cedited by Bernd Sokolowski.
260 _aNew York, N.Y. :
_bHumana ;
_a[London :
_bSpringer, distributor],
_cc2009.
300 _axii, 421 p. :
_bill. (some col.) ;
_c27 cm.
490 1 _aSpringer protocols
490 1 _aMethods in molecular biology,
_x1064-3745 ;
_v493
504 _aIncludes bibliographical references and index.
505 0 _aRNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio ... [et al.] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande ... [et al.] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico ... [et al.] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan ... [et al.] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith ... [et al.] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada.
650 0 _aVestibular apparatus.
650 0 _aHearing disorders.
650 0 _aEquilibrium (Physiology)
650 2 _aAuditory Pathways
_xphysiology.
650 2 _aEar Diseases
_xdiagnosis.
650 2 _aVestibule, Labyrinth
_xphysiology.
650 2 _aVestibular Diseases
_xdiagnosis.
700 1 _aSokolowski, Bernd.
830 0 _aSpringer protocols.
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 493.
906 _a7
_bcbc
_ccopycat
_d2
_encip
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942 _2ddc
_cSTS