Understanding DNA and gene cloning : a guide for the curious /
Karl Drlica.
- 4th ed.
- Hoboken, NJ : Wiley, c2004.
- xiv, 369 p. : ill. ; 23 cm.
Includes bibliographical references (p. 325-335) and index.
Chapter 1. Preview: Life as Interacting Molecules. PART I. BASIC MOLECULAR GENETICS. Chapter 2. Structure of DNA: Two Long, Interwound Chains. Chapter 3. Reproducing DNA: Information Transfer from One Generation to the Next. Chapter 4. Gene Expression: Cellular Use of Genetic Information. PART II. MANIPULATION DNA. Chapter 5. Microbial Growth: One-celled Organisms as Tools for Gene Cloning. Chapter 6. Plasmids and Phages: Submicroscopic Parasites Used to Deliver Genes to Cells. Chapter 7. Cutting and Joining DNA: Restriction Endonucleases and Ligases Used to Restructure DNA Molecules. Chapter 8. Using Complementary Base Pairing: Hybridization, Probes, and Amplification using PCR. Chapter 9. Cloning Genes: Isolation of a Hemoglobin Gene. Chapter 10. Using Cloned Genes: Gene Structure, Expression Vectors, and Gene Function. PART III. MODERN MOLECULAR GENETICS. Chapter 11. Beyond the Central Dogma: A Sampling of Insights Derived from Gene Cloning. Chapter 12. Retroviruses: AIDS and Gene Delivery. Chapter 13. Cancer Genes: The Loss of Growth Control. PART IV: HUMAN GENETICS. Chapter 14. Heredity: Pattern of Inheritance and Genetic Instability. Chapter 15. Application of Human Genetics: Genetic Testing, Gene Therapy, and DNA Fingerprinting. PART V. WHOLE GENOMES. Chapter 16. Genomics: Using Whole Genomes to Study Life. Additional Reading. Glossary. Abbreviations. Index.